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Accumulated citrate is exported to the cytoplasm where it is catalyzed by ATP citrate lyase (ACLY) to generate cytosolic acetyl-CoA which is a key precursor of fatty acids. Acetyl-CoA is then carboxylated by ACC to synthesize malonyl-CoA which is then converted to palmitate (16-carbon saturated fatty acid) as the first fatty acid in lipogenesis by the key rate limiting enzyme (123). ACC and FAS are both highly expressed in the embryonic cell and the functions of both enzymes are essential for development.]